Evaluation of antifungal activity of novel marine actinomycete, Streptomyces sp. AA13 isolated from sediments of Lake Oubeira (Algeria) against Candida albicans

Adel, Ayari and Houda, Morakchi and Djamila, Kirane Gacemi (2016) Evaluation of antifungal activity of novel marine actinomycete, Streptomyces sp. AA13 isolated from sediments of Lake Oubeira (Algeria) against Candida albicans. African Journal of Microbiology Research, 10 (6). pp. 156-171. ISSN 1996-0808

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Abstract

A new actinomycete strain, designated AA13 was isolated from a marine sediment sample obtained from Lake Oubeira, in the North-East of Algeria and selected for its antifungal activities against Candida albicans. Morphological, physiological and biochemical properties and 16S rRNA gene sequencing strongly suggested that this strain was a new species, which belonged to the genus Streptomyces. Study of the influence of different nutritional compounds and culture conditions on growth and production of compounds with antifungal activity by the Streptomyces sp. strain AA13 indicated that the highest biomass and biological activities were obtained by utilizing the glycerol and peptone as carbon and nitrogen sources, respectively, with pH 7.0 and incubation temperature of 30°C. Two bioactive spots were detected by analysis of the ethyl acetate extract by thin-layer chromatography (TLC) and bioautography analyses. Among these bioactive compounds (antibiotics), a complex AA13-B that showed the interesting antifungal activity, was selected and purified by high performance liquid chromatography (HPLC), which indicated the presence of three peaks. Interestingly, the infrared spectroscopy (IR) studies showed that the molecule AA13-B2 contain an aromatic ring substituted by aliphatic chains. However, the investigations which determine the structure of the antifungal molecule are in progress.

Item Type: Article
Subjects: Science Global Plos > Biological Science
Depositing User: Unnamed user with email support@science.globalplos.com
Date Deposited: 05 Apr 2023 08:12
Last Modified: 29 Feb 2024 04:26
URI: http://ebooks.manu2sent.com/id/eprint/468

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