Galectin-3 expression favors metastasis in murine melanoma

Comodo, Andréia Neves and Lacerda Bachi, Andre Luis and Soares, Maria Fernanda and Franco, Marcello and Castro Teixeira, Vicente de Paulo (2013) Galectin-3 expression favors metastasis in murine melanoma. Advances in Bioscience and Biotechnology, 04 (10). pp. 55-62. ISSN 2156-8456

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Abstract

Galectin-3 is a member of the lectin family that binds β-galactosides and plays an important role in several types of tumors. Melanoma is an invasive cancer responsible for 80% of deaths associated to skin cancers. There are some evidences that galectin-3 interacts with β-catenin, a molecule involved with Wnt signaling pathway. Here, we evaluate the role of galectin-3 intumor growth and metastasis, as well as its interaction with β-catenin. Murine melanoma cells (B16F10) were injected subcutaneously and intravenously in male C57BL/6 wild-type (WT) and galectin-3 knock-out (KO) mice. Tumor growth and lung melanoma colonies were assessed. The expression of galectin-3 and β-catenin was evaluated by immuno-histochemistry. We observed that tumor growth did not differ between the groups. However, to metastasis, the number of lung colonies in WT mice was significantly increased in comparison to that observed in KO mice. The cytoplasm expression of galectin-3 was observed in subcutaneous and metastatic tumors,w in both groups. We observed its nuclear expression in some of subcutaneous tumors of KO mice. The expression of β-catenin was detected in cell membrane of all subcutaneous tumors analyzed, whereas in the metastatic tumors we observed both cytoplasm and cell membrane staining. Altogether, our data suggest that galectin-3 favors the metastasis of melanoma cells and this process is not associated with β-catenin.

Item Type: Article
Subjects: Science Global Plos > Biological Science
Depositing User: Unnamed user with email support@science.globalplos.com
Date Deposited: 18 Mar 2023 10:18
Last Modified: 18 Mar 2024 03:56
URI: http://ebooks.manu2sent.com/id/eprint/376

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