Evaluation of in vivo migration of chondrocytes from tissue-engineered cartilage that was subcutaneously transplanted in mouse model

Matsuyama, Mariko and Fujihara, Yuko and Inaki, Ryoko and Nishizawa, Satoru and Nagata, Satoru and Takato, Tsuyoshi and Hoshi, Kazuto (2013) Evaluation of in vivo migration of chondrocytes from tissue-engineered cartilage that was subcutaneously transplanted in mouse model. Open Journal of Regenerative Medicine, 02 (04). pp. 93-98. ISSN 2169-2513

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Abstract

For regenerative medicine, clarification of in vivo migration of transplanted cells is an important task to secure the safety of transplanted tissue. We had prepared tissue-engineered cartilage consisting of cultured chondrocytes with collagen hydrogel and a biodegradable porous polymer, and we clinically applied it for treatment of craniofacial anomaly. To verify the safety of this tissue-engineered cartilage, we had syngenically transplanted the tissue-engineered cartilage using chondrocytes harvested from EGFP-transgenic mice into subcutaneous pocket of wild type mice, and investigated localizations of transplanted chondrocytes in various organs including cerebrum, lung, liver, spleen, kidney, auricle, gastrocnemius, and femur. After 8 to 24 weeks of the transplantation, accumulation of cartilaginous matrices was observed in tissue-engineered cartilage, while EGFP-positive transplanted chondrocytes were localized in this area. Otherwise, no EGFP was immunohistochemically detected in each organ, suggesting that subcutaneously-transplanted chondrocytes do not migrate to other organs through the circulation. In cartilage tissue engineering using cultured chondrocytes, risk for migration and circulation of transplanted cells seemed negligible, and that ectopic growth of the cells was unlikely to occur, showing that this is safe technique with regard to the in vivo migration of transplanted cells.

Item Type: Article
Subjects: Science Global Plos > Biological Science
Depositing User: Unnamed user with email support@science.globalplos.com
Date Deposited: 15 Mar 2023 12:54
Last Modified: 24 Feb 2024 04:25
URI: http://ebooks.manu2sent.com/id/eprint/315

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