Assessment of Genetic Diversity in Tomato (Solanum lycopersicum) Varieties by Using RAPD Markers

Katad, P. U. and Jadhav, A. U. and Surbhaiyya, S. D. and Jagtap, K. A. (2024) Assessment of Genetic Diversity in Tomato (Solanum lycopersicum) Varieties by Using RAPD Markers. Journal of Advances in Biology & Biotechnology, 27 (8). pp. 260-267. ISSN 2394-1081

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Abstract

Aim: Assessment of genetic diversity in Tomato (Solanum lycopersicum) varieties by using RAPD markers.

Place and Duration of Study: Department of Plant Biotechnology of K. K. Wagh College of Agricultural Biotechnology, Nashik, Maharashtra.

Methodology: Tomato is one of the most important vegetable crops in the world and normally human being daily consumed in diet. The five Varities were collected from Mahalaxmi Hi-Tech Nursery Nashik. The present study was conducted at K K Wagh college of Agricultural Biotechnology Nashik. DNA was isolated by using modified CTAB method Five Varities were analyzed through RAPD markers to determine the molecular characterization at individual level.

Results: Six arbitrary oligonucleotide primers used in the RAPD-PCR produced a total 38 different marker bands with an average 8 band per primer. Based on the banding pattern 100% polymorphism observed among the tomato varieties. Size range of amplified DNA bands varied from 0.1-10 kb. A total of 14 unique bands were amplified from the genome of the 5 tomato varieties. The dendrogram clustered the tomato varieties into two major clusters. The clusters were subdivided into small groups including the highly related Keshar and 0225, Anisha and Sai-22 varieties. The other Cluster included an isolated variety of Aryaman as a distinctive one. The RAPD data revealed a high genetic polymorphism that can be used to select diverse parents in breeding programs and maintain genetic variation.

Item Type: Article
Subjects: Science Global Plos > Biological Science
Depositing User: Unnamed user with email support@science.globalplos.com
Date Deposited: 18 Jul 2024 10:25
Last Modified: 18 Jul 2024 10:25
URI: http://ebooks.manu2sent.com/id/eprint/2725

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