A phage-displayed disulfide constrained peptide discovery platform yields novel human plasma protein binders

Gao, Xinxin and Kaluarachchi, Harini and Zhang, Yingnan and Hwang, Sunhee and Hannoush, Rami N. and De March, Matteo (2024) A phage-displayed disulfide constrained peptide discovery platform yields novel human plasma protein binders. PLOS ONE, 19 (3). e0299804. ISSN 1932-6203

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Abstract

Disulfide constrained peptides (DCPs) show great potential as templates for drug discovery. They are characterized by conserved cysteine residues that form intramolecular disulfide bonds. Taking advantage of phage display technology, we designed and generated twenty-six DCP phage libraries with enriched molecular diversity to enable the discovery of ligands against disease-causing proteins of interest. The libraries were designed based on five DCP scaffolds, namely Momordica charantia 1 (Mch1), gurmarin, Asteropsin-A, antimicrobial peptide-1 (AMP-1), and potato carboxypeptidase inhibitor (CPI). We also report optimized workflows for screening and producing synthetic and recombinant DCPs. Examples of novel DCP binders identified against various protein targets are presented, including human IgG Fc, serum albumin, vascular endothelial growth factor-A (VEGF-A) and platelet-derived growth factor (PDGF). We identified DCPs against human IgG Fc and serum albumin with sub-micromolar affinity from primary panning campaigns, providing alternative tools for potential half-life extension of peptides and small protein therapeutics. Overall, the molecular diversity of the DCP scaffolds included in the designed libraries, coupled with their distinct biochemical and biophysical properties, enables efficient and robust identification of de novo binders to drug targets of therapeutic relevance.

Item Type: Article
Subjects: Science Global Plos > Biological Science
Depositing User: Unnamed user with email support@science.globalplos.com
Date Deposited: 06 Apr 2024 13:20
Last Modified: 06 Apr 2024 13:20
URI: http://ebooks.manu2sent.com/id/eprint/2584

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