Isolation and Functional Characterization of a Constitutive Promoter in Upland Cotton (Gossypium hirsutum L.)

Yang, Yang and Li, Xiaorong and Li, Chenyu and Zhang, Hui and Tuerxun, Zumuremu and Hui, Fengjiao and Li, Juan and Liu, Zhigang and Chen, Guo and Cai, Darun and Chen, Xunji and Li, Bo (2024) Isolation and Functional Characterization of a Constitutive Promoter in Upland Cotton (Gossypium hirsutum L.). International Journal of Molecular Sciences, 25 (3). p. 1917. ISSN 1422-0067

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Abstract

Multiple cis-acting elements are present in promoter sequences that play critical regulatory roles in gene transcription and expression. In this study, we isolated the cotton FDH (Fiddlehead) gene promoter (pGhFDH) using a real-time reverse transcription-PCR (qRT-PCR) expression analysis and performed a cis-acting elements prediction analysis. The plant expression vector pGhFDH::GUS was constructed using the Gateway approach and was used for the genetic transformation of Arabidopsis and upland cotton plants to obtain transgenic lines. Histochemical staining and a β-glucuronidase (GUS) activity assay showed that the GUS protein was detected in the roots, stems, leaves, inflorescences, and pods of transgenic Arabidopsis thaliana lines. Notably, high GUS activity was observed in different tissues. In the transgenic lines, high GUS activity was detected in different tissues such as leaves, stalks, buds, petals, androecium, endosperm, and fibers, where the pGhFDH-driven GUS expression levels were 3–10-fold higher compared to those under the CaMV 35S promoter at 10–30 days post-anthesis (DPA) during fiber development. The results indicate that pGhFDH can be used as an endogenous constitutive promoter to drive the expression of target genes in various cotton tissues to facilitate functional genomic studies and accelerate cotton molecular breeding.

Item Type: Article
Subjects: Science Global Plos > Multidisciplinary
Depositing User: Unnamed user with email support@science.globalplos.com
Date Deposited: 06 Feb 2024 06:07
Last Modified: 06 Feb 2024 06:07
URI: http://ebooks.manu2sent.com/id/eprint/2476

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