Study of Androgenesis using Morphometrics and Regeneration in Indian Mustard (Brassica juncea Czern. and Coss.)

Anther culture involves identification, isolation and culture of appropriate stage of microspore, which world induce callus /embryo and subsequently give rise to androgenic plant. Anther culture of yellow seeded mustard (Brassica juncea) was carried out using floral morphometrices. It was observed that bud length has greater correlation with anther elongation/development than other floral parts. Development of microspore derived callus and regeneration through embryogenesis exhibited intermediate structures, which was observed through histology and scanning electron microscopy. Cellular and tissue organization for haploid tissue regeneration was similar to zygotic embryo formation; asynchronous development occurred in different region of the same calli.