EVALUATION OF PHYTOCHEMICALS, ANTIMICROBIAL, ANTIOXIDANT AND DNA DAMAGE PROTECTION ACTIVITY FROM In-vitro CULTURE EXTRACTS OF Wrightia tinctoria (Roxb.)

Wrightia tinctoria (Roxb.) was a endangered plant having medicinal importance belongs to the Apocynacea family. The aim was to investigate the preliminary phytochemical analysis, antibacterial activity, antioxidant of seed, leaf and callus extracts of Wrightia tinctoria. The study was done for fifteen days old germinated plant of Cotyledon leaf explants on the ½ MS medium BAP (1 mg/l) + NAA (0.5 mg/l) used for the initiation of callus for further preparations. Activity dividing cells was produced in 6th week, which was friable greenish and predominant callus. The bioactive compounds was used for antibacterial, antioxidant activities and the phytochemical analysis of seed, leaf and callus predicted the presence of phytochemical constituents such as carbohydrates, phenols, phytosterols, saponins, alkaloids, xanthoproteins, quinones, tannins, starch, coumarin, triterpenoids and flavonoids. The antibacterial activity of a crude aqueous extract of callus, leaf and seed tested against 15 different bacterial strains by agar well diffusion method. The Comparative study of antibacterial activity reveals that callus extract of Wrightia tinctoria illustrated a wide spectrum that inhibited the growth of microorganisms with a maximum inhibition zone of about 20 mm against Enterobacter aerogenes. The DPPH free radical scavenging activity of callus extract of Wrightia tinctoria was shown higher percentage. The different extracts of Wrightia tinctoria were significantly protected the DNA damage against the oxidation by Fenton’s reaction. From the above data resulted, the callus extract of Wrightia tinctoria was found to have potential in treating human ailments, and thus pave the way for the formulation of new potent drugs.