ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF Celastrus paniculatus MEDIATED COPPER NANOPARTICLES

Aim: The aim of the study is to evaluate Celastrus paniculatus mediated copper nanoparticles and their antimicrobial and antioxidant activity

Introduction: C. paniculatus is a member of the Celastraceae family and is a large, woody climber with a yellow, corky bark. It grows throughout India, and has been reported to grow as tall as almost 2000 m. The plants exhibit varying degrees of therapeutic values, some of which are its use in the treatment of cognitive dysfunction, epilepsy, insomnia, rheumatism, gout, dyspepsia. Oil from the seeds called as Jyothismati oil, which contains the alkaloids celastrine and paniculatin in varying amounts, is the most commonly used plant part.

Materials And Methods: The agar well diffusion method was used to determine the antibacterial activity of copper nanoparticles. Different concentrations of CuNPs were tested against Streptococcus mutans, Candida albicans and Lactobacillus spp. The antibiotics were used as positive control. Zone of inhibition was recorded in each plate. For antioxidant activity 50% methanol, DPPH solution and CuNP was added in 5 test tubes ranging from 10 - 50 microliters and kept in a dark place for 10 minutes and the reading was noted

Results: As a result, there is a dose dependent gradual increase of activity on S. mutans, C. albicans, Lactobacillus spp. at 150 microliters when compared to the standard and antioxidant activity was seen at 50 microliters where the percentage of inhibition was maximum.

Conclusion: As a result, it is conclude that C. paniculates mediated copper nanoparticles has a high antimicrobial and antifungal activity also, it has a good antioxidant effect.