Coordinated circadian timing through the integration of local inputs in Arabidopsis thaliana

Greenwood, Mark and Domijan, Mirela and Gould, Peter D. and Hall, Anthony J. W. and Locke, James C. W. and Estelle, Mark (2019) Coordinated circadian timing through the integration of local inputs in Arabidopsis thaliana. PLOS Biology, 17 (8). e3000407. ISSN 1545-7885

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Abstract

Individual plant cells have a genetic circuit, the circadian clock, that times key processes to the day-night cycle. These clocks are aligned to the day-night cycle by multiple environmental signals that vary across the plant. How does the plant integrate clock rhythms, both within and between organs, to ensure coordinated timing? To address this question, we examined the clock at the sub-tissue level across Arabidopsis thaliana seedlings under multiple environmental conditions and genetic backgrounds. Our results show that the clock runs at different speeds (periods) in each organ, which causes the clock to peak at different times across the plant in both constant environmental conditions and light-dark (LD) cycles. Closer examination reveals that spatial waves of clock gene expression propagate both within and between organs. Using a combination of modeling and experiment, we reveal that these spatial waves are the result of the period differences between organs and local coupling, rather than long-distance signaling. With further experiments we show that the endogenous period differences, and thus the spatial waves, can be generated by the organ specificity of inputs into the clock. We demonstrate this by modulating periods using light and metabolic signals, as well as with genetic perturbations. Our results reveal that plant clocks can be set locally by organ-specific inputs but coordinated globally via spatial waves of clock gene expression.

Item Type: Article
Subjects: Science Global Plos > Biological Science
Depositing User: Unnamed user with email support@science.globalplos.com
Date Deposited: 27 Jan 2023 08:57
Last Modified: 28 Sep 2023 09:29
URI: http://ebooks.manu2sent.com/id/eprint/21

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