Assessment of Pepper Pungency Using Real Time PCR to Evaluate the Pun1 AND Catf2 Genes Expression in Capsicum Annuum Varieties of Takanotsume Hot Pepper and Yellow Bell Pepper

Tiwari, Shishir and Sao, Shweta and Kurrey, Antu (2023) Assessment of Pepper Pungency Using Real Time PCR to Evaluate the Pun1 AND Catf2 Genes Expression in Capsicum Annuum Varieties of Takanotsume Hot Pepper and Yellow Bell Pepper. UTTAR PRADESH JOURNAL OF ZOOLOGY, 44 (2). pp. 64-74. ISSN 0256-971X

Full text not available from this repository.

Abstract

The accumulation of the alkaloid capsaicin and its derivatives in Capsicum fruits results in their pungent flavor. A RT-PCR assay was used to examine the pungency levels of Capsicum annuum species by employing gene-specific primers associated with the capsaicin biosynthetic pathway. The expression of the Acyl-transferase encoded by the Pun1 and Catf2 was directly related to capsaicin accumulation. Low-abundance mRNA transcripts were measured with RT-PCR. Pun1 and Catf2 gene expression was found to be correlated with the phenotypic trait (pungency level) in our study, and has a useful method for distinguishing between Capsicum species' pungency levels. The RT-PCR results showed that the candidate capsaicin biosynthetic genes (Pun1, and Catf2) were present in Capsicum annuum species with high and moderate levels of pungency, whereas the expression levels of these two genes were low in yellow bell pepper (Non-Pungent) with low levels of pungency and high expression level was measured in Takanotsume Hot Pepper variety. In conclusion, gene specific PCR data, gene expression profile and volatile compounds analysis collectively indicate that the Pun1 and Catf2 genes in pepper encodes a acyltransferase. The Pun1 and Catf2 genes in pepper had crucial role in pungency level of peppers.

Item Type: Article
Subjects: Science Global Plos > Biological Science
Depositing User: Unnamed user with email support@science.globalplos.com
Date Deposited: 06 Nov 2023 04:55
Last Modified: 06 Nov 2023 04:55
URI: http://ebooks.manu2sent.com/id/eprint/1925

Actions (login required)

View Item
View Item