Effects of Vitamin B12 Deficiency on Amyloid-β Toxicity in Caenorhabditis elegans

Andra, Arif and Tanigawa, Shoko and Bito, Tomohiro and Ishihara, Atsushi and Watanabe, Fumio and Yabuta, Yukinori (2021) Effects of Vitamin B12 Deficiency on Amyloid-β Toxicity in Caenorhabditis elegans. Antioxidants, 10 (6). p. 962. ISSN 2076-3921

[thumbnail of antioxidants-10-00962-v2.pdf] Text
antioxidants-10-00962-v2.pdf - Published Version

Download (2MB)

Abstract

High homocysteine (Hcy) levels, mainly caused by vitamin B12 deficiency, have been reported to induce amyloid-β (Aβ) formation and tau hyperphosphorylation in mouse models of Alzheimer’s disease. However, the relationship between B12 deficiency and Aβ aggregation is poorly understood, as is the associated mechanism. In the current study, we used the transgenic C. elegans strain GMC101, which expresses human Aβ1–42 peptides in muscle cells, to investigate the effects of B12 deficiency on Aβ aggregation–associated paralysis. C. elegans GMC101 was grown on nematode growth medium with or without B12 supplementation or with 2-O-α-D-glucopyranosyl-L-ascorbic acid (AsA-2G) supplementation. The worms were age-synchronized by hypochlorite bleaching and incubated at 20 °C. After the worms reached the young adult stage, the temperature was increased to 25 °C to induce Aβ production. Worms lacking B12 supplementation exhibited paralysis faster and more severely than those that received it. Furthermore, supplementing B12-deficient growth medium with AsA-2G rescued the paralysis phenotype. However, AsA-2G had no effect on the aggregation of Aβ peptides. Our results indicated that B12 supplementation lowered Hcy levels and alleviated Aβ toxicity, suggesting that oxidative stress caused by elevated Hcy levels is an important factor in Aβ toxicity.

Item Type: Article
Subjects: Science Global Plos > Agricultural and Food Science
Depositing User: Unnamed user with email support@science.globalplos.com
Date Deposited: 15 Jul 2023 05:46
Last Modified: 07 Oct 2023 10:06
URI: http://ebooks.manu2sent.com/id/eprint/1404

Actions (login required)

View Item
View Item