Preparation of Ethanolic Extract of Cassia auriculata and Its Anti-Diabetic Activity

Sitaram, T. Srinivasa Surya and Arivarasu, Lakshminarayanan and Rajeshkumar, S. and Thangavelu, Lakshmi (2021) Preparation of Ethanolic Extract of Cassia auriculata and Its Anti-Diabetic Activity. Journal of Pharmaceutical Research International, 33 (62A). pp. 380-386. ISSN 2456-9119

[thumbnail of 5346-Article Text-7470-1-10-20221006.pdf] Text
5346-Article Text-7470-1-10-20221006.pdf - Published Version

Download (372kB)

Abstract

Introduction: Diabetes has caused a major burden to the health sector in the developing countries and has shown an increasing trend among the urban population. It is estimated that most patients are with type II diabetes which could be easily treated with dietary changes, exercise, and medication. Sri Lanka carries a long history of ayurvedic medicine where it uses the plant for treating many diseases. Therefore it is important to screen medicinal plants scientifically so they could be used safely and effectively in the traditional medical system and also be used for further investigations. Cassia auriculata is a plant used in the Ayurvedic medical system in Sri Lanka for treating many diseases including diabetics. We evaluated the anti-diabetic properties and the antioxidant properties of Cassia auriculata leaves.

Methods: The methanol extract of the leaves was sequentially extracted with petroleum ether and thereafter was partitioned between EtOAc, and water. The α-amylase inhibition assay was performed using the 3,5- dinitrosalicylic acid method. The antioxidant activities were measured using the DPPH free radical scavenging activity and the total phenolic content using Folin-Ciocalteu’s reagent. The cytotoxicity of the extract was evaluated using the Brine shrimp bioassay.

Results: The extract shows very good anti-diabetic activity for the Cassia auriculata extract by using BSA and EAA Assay.

Conclusion: The leaf extracts of cassia auriculata exhibit remarkable α-amylase inhibitory activity in the crude methanolic extract. Hence leaves of cassia auriculata have a potential to be used as a regular green vegetable and also be investigated further in isolating pure compounds with anti-diabetic activity.

Item Type: Article
Subjects: Science Global Plos > Medical Science
Depositing User: Unnamed user with email support@science.globalplos.com
Date Deposited: 31 Jan 2023 08:52
Last Modified: 16 Sep 2023 05:54
URI: http://ebooks.manu2sent.com/id/eprint/104

Actions (login required)

View Item
View Item