Performance of Short Chain Length Polyhydroxyalkanoate Production by Comamonas sp EB172 in Large Scale Bioreactors Based on Constant Impeller Tip Speed

Mumtaz, Tabassum and Mohammadi, Mitra and Yee, Lian-Ngit and Abd-Aziz, Suraini and Yee, Phang and Ariffin, Hidayah and Shirai, Yoshihito and Hassan, Mohd (2016) Performance of Short Chain Length Polyhydroxyalkanoate Production by Comamonas sp EB172 in Large Scale Bioreactors Based on Constant Impeller Tip Speed. British Biotechnology Journal, 13 (2). pp. 1-11. ISSN 22312927

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Abstract

Aims: In this study, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) or PHBV, a short chain length polyhydroxyalkanoates (PHAs) production by a local isolate Comamonas sp. EB172 using 500 g/L mixed organic acids were investigated in 10 L and 150 L stirred tank bioreactors based on constant impeller tip speed (minimum 0.56 m/s and maximum 2.22 m/s).

Results: The ratio of the three acids i.e., acetic, butyric, and propionic, were 2:1:1, mimicking clarified mixed acids obtained from anaerobically treated palm oil mill effluent. In pH controlled fed-batch cultivation, in 10 L bioreactor, the strain could accumulate 84% PHBV with biomass concentration of about 4.08 g/L under dual nutrient limitation strategy i.e., dissolved oxygen and nitrogen limited conditions. By maintaining similar tip speed in 150 L bioreactor, a final biomass concentration of 5.35 g/L with a PHBV content of 72% and volumetric productivity of 0.083 g/L/h were obtained. The calculated yield was 0.259 g PHBV/g mixed acids.

Conclusion: Both the yield and PHA content in 150 L bioreactor were comparable to that obtained in 10 L scale where PHBV content ranged from 70- 90% (w/w) of the cell with yield of 0.27- 0.4 g PHBV/g mixed acid, respectively. This is the first report of utilizing mixed organic acids for PHBV biosynthesis in large scale.

Item Type: Article
Subjects: Science Global Plos > Biological Science
Depositing User: Unnamed user with email support@science.globalplos.com
Date Deposited: 23 May 2023 08:17
Last Modified: 25 Jan 2024 04:15
URI: http://ebooks.manu2sent.com/id/eprint/936

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