Moghtaderi, Hassan and Sepehri, Houri and Delphi, Ladan and Attari, Farnoosh (2018) Gallic acid and curcumin induce cytotoxicity and apoptosis in human breast cancer cell MDA-MB-231. BioImpacts, 8 (3). pp. 185-194. ISSN 2228-5660
bi-8-185.pdf - Published Version
Download (2MB)
Abstract
Introduction: Gallic acid (GA) and curcumin (Cur) are natural phenolic compounds that their anti-tumor effects on many types of cancers have been proved. In the current study, the effect of the combination of these agents on MDA-MB-231 breast cancer cells was investigated.
Methods: Inhibition of cell proliferation (MTT assay), light microscopy, fluorescence microscopy, cell cycle analysis, nitrite detection, ROS levels, measurement of the mitochondrial membrane potential, GSH level, Annexin V assay, RT-PCR and Western blotting methods were applied.
Results: The results revealed the combination of GA and Cur strongly decreased MDA-MB-231 cell growth. Moreover, this combination increased ROS level and cytotoxic activity along with the glutathione depletion in MDA-MB-231 cells. Flow cytometry analysis showed the combination of GA and Cur increased sub-G1 cell population. Furthermore, fluorescent staining and Annexin V/PI assay showed that apoptotic cells were significantly increased in the presence of GA and Cur. At last, protein expression evaluation showed that the combination of GA and Cur significantly decreased Bcl-2 level while increased Bax, cleaved-caspase3 and PARP levels in MDA-MB-231 cells.
Conclusion: These results suggest that GA in combination with Cur could be a possible candidate for chemoprevention agent of triple negative breast cancer.
Item Type: | Article |
---|---|
Subjects: | Science Global Plos > Medical Science |
Depositing User: | Unnamed user with email support@science.globalplos.com |
Date Deposited: | 31 Mar 2023 08:14 |
Last Modified: | 19 Sep 2023 07:54 |
URI: | http://ebooks.manu2sent.com/id/eprint/432 |