Determination of Analgesic Potential of Dibenzylidene Derivatives of Cyclohexanone

Background: Pain is significantly associated with most disease states, and the search for suitable analgesic alternatives with fewer side effects is continuous and urgent.

Aim of the study: This study is aimed at the evaluation of the analgesic potential of cyclohexanone derivatives which include; 2, 6 (P-dimethylaminobenzylidene) cyclohexanone, 2,6-bis[(4-methoxyphenyl) methylidene] cyclohexan-1-one, 2,6-diethylidenecyclohexan-1-one, 2,6-dibenzylidenecyclohexan-1-one, and 2,6-dibenzodioxylmethylidenecyclohexan-1-one (D1-D5 respective).

Methodology: The study measured analgesia potential using the hot plate and tail flick model. The mice were divided into five groups, (GP): GP I and V were control group (0.2 ml/kg distilled water) and standard group (50 mg/kg Tramadol Hydrochloride) respectively, GP II to IV were administered the different doses (500, 1000, and 1500 mg/kg) of the test compounds respectively, and evaluated 30 min afterward. The latency to pain was observed at 30 minutes, 60 minutes and 90 minutes at doses of 500, 1000, and 1500 mg/kg respectively.

Results: The hot plate model showed significance in pain inhibition with D1 proven 45.5 %, 59% at 60 min,90 min respectively, and a significant (p < 0.0008) increase in latency to pain at 90 mins with the dose of 1500 mg/kg. D5 proven 45.2%, 48.9%, and 79.8% pain inhibition at 30, 60 and 90 min respectively with 500 mg/kg; 75.4%, 71.9%, and 85.9% pain inhibition at 30,60 and 90 min respectively with 1000 mg/kg; 40.1%, 65.5% and 80.5% pain inhibition at 30, 60 and 90 min respectively with 1500 mg/kg and significant (p < 0.0001) increase latency to pain at 90 minutes with the doses, 500 mg/kg, 1000 mg/kg, and 1500 mg/kg.

Conclusion: The study gave an insight into the analgesic potential of the Cyclohexanone derivatives. D1 and D5 derivatives exhibited remarkable analgesic potential in the hot plate model, and the study outcomes suggest their useability as adjuvants in the management of pain, especially in veterinary patients.