Analytical Method Development and Validation of Triamcinolone Acetonide by Using RP-HPLC Technique

The aim of this study primarily focuses on the analytical Method Development and Validation of Triamcinolone Acetonide by using RP-HPLC Technique. Triamcinolone acetonide is a Glucocorticosteroid with great immunosuppressive and anti-inflammatory activity used in the treatment of redness, itching, scaling, crusting like inflammatory skin disorders, and other conditions like dryness with psoriasis on the skin. The proposed method is an economic and highly sensitive, Reverse Phase High Performance Liquid Chromatographic method for quantification of Triamcinolone acetonide is an active Pharma ingredient (API). The efficient separation was done by using X-bridge phenyl column has a stationary phase and a mobile phase of 50:50 v/v of acetonitrile with Octane sulphonic acid as a buffer solution to adjust the desirable pH-2.5 condition by employing ortho phosphoric acid. The efficient separation of Triamcinolone acetonide was eluted at 6.22 min and its UV absorption was done at 235 nano meters at input flow rate of 1ml/min with Photodiode Array Detector. All the results are acceptable limits with respect to ICH guidelines and the linearity results of Triamcinolone acetonide gave regression equation y= 43591x+14344 and correlation coefficient is (R2) 0.9997. The % RSD is 0.24 for intra-day precision and 0.65 for inter-day precision supports the ruggedness and the proposed approach is eco-friendly by AGREE-Analytical GREEnness metric shows 0.72. The quantification and appropriateness of the method was studied by the various testing parameters like recovery test, fidelity, accuracy, Robustness, and stability of the solution, one can understand the correctness of the approach.