Phytochemical Screening, Antimicrobial Potential and GC-MS Analysis of Chloroform Leaf Extract of Hyptis suaveolens (L.) Poit
M. Murugan *
Sri Kaliswari College (Autonomous), Sivakasi, Virudhunagar District, Tamil Nadu, India.
D. Sheeba Gnanadeebam
Gobi Arts and Science College, Gopichettipalayam, Erode District, Tamil Nadu, India.
G. Varatharaju
Sri Kaliswari College (Autonomous), Sivakasi, Virudhunagar District, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Bioactive compounds possessing therapeutic value are used for long time by vast group of researchers to treat various diseases for the goodness of the mankind. Most of the commercially available bioactive compounds are derived from plants because of their affirmative property on human health with minimum side effects. Hyptis suaveolens (L.) Poit. is one of the most important traditional and therapeutic plant, which is belongs to Lamiaceae family. The Biochemical test of the leaf extract of Hyptis suaveolens using different solvent shows the presence of biologically significant compounds. Thereby, the Antimicrobial properties of leaf extract of H. suaveolens carried out against six different bacterial pathogens and three fungi pathogens. Most potential activity was observed in the crude extract of Hexane, Ethyl acetate and Chloroform against E.coli, Enterococcus faecalis and Streptococcus pyrogenes respectively when compared to Klebshilla pneumonia. Among the other crude extract, the chloroform crude extract resulted with more number of molecules in the phytochemical analysis and also it exhibits superior antibacterial activity. Hence, the Gas Chromatography-Mass Spectrometry (GC-MS) analysis of chloroform extract was achieved and 17 different bioactive compounds were discovered. Neophytadiene, (S,Z)-Heptadeca-1,9-dien-4,6-diyn-3-ol, 2,3-Diazabicyclo[2.2.1]hept-2-ene, 7,7-dime and Stigmasterol are the significant molecules present in the chloroform leaf extract it has the highest value for the mankind.
Keywords: GC-MS, antimicrobial property, phytochemical analysis, medicinal plant and hyptis suaveolens
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